phenol chloroform isoamyl alcohol preparation guide

Chloroform

Chloroform is a colorless volatile liquid derivative of trichloromethane with an ether-like odor Formerly used as an inhaled anesthetic during surgery the primary use of chloroform today is in industry where it is used as a solvent and in the production of the refrigerant freon

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Chloroform

Chloroform is a colorless volatile liquid derivative of trichloromethane with an ether-like odor Formerly used as an inhaled anesthetic during surgery the primary use of chloroform today is in industry where it is used as a solvent and in the production of the refrigerant freon

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RNA Reference Guide

R NA reference guide Sample preparation and purification solutions Printed on recycled paper 2 For over three decades and published in over 100 000 publications scientists have trusted their most precious samples with our innovative and robust RNA isolation and purification products

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Phenol / Chloroform / Isoamyl Alcohol 1 Phase

Amresco's ready-to-use single-phase solutions do not have to be equilibrated with a buffering purification solution prior to use These phenol products for nucleic acid purification allow easier sample preparation and reduce the hazards associated with preparing traditional phenol solutions in the laboratory

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A Simple Method of Genomic DNA Extraction from Human

10-4-2020We developed a rapid cost-effective and noninvasive method of sample collection and simple DNA extraction from buccal swabs urine and hair using the phenol-chloroform method Buccal samples were subjected to DNA extraction immediately or after refrigeration (4–6C) for 3 days

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Total RNA isolation protocol

The procedure is suitable for all types of tissues from wide variety of animal (and blood) and plant species All steps are performed at room temperature (RT) (without ice) and without DEPC-treated water RNA precipitate with lithium chloride (LiCl) for increased stability of the RNA preparation and improvement of cDNA synthesis

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A descriptive guide for absolute quantification of

A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR add nuclease-free water to the digested plasmids (final volume 100 l) and one volume of phenol:chloroform:isoamyl alcohol Vortex and centrifuge (15000 g 2 min) at room temperature

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RNA Purification

library construction and preparation of labeled cDNA for hybridization to gene arrays To avoid generating cDNA libraries with large numbers of ribosomal clones and nonspecific labeled cDNA it is crucial to start with poly(A) RNA for these procedures The next section gives a

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A Simple Method of Genomic DNA Extraction from Human

10-4-2020We developed a rapid cost-effective and noninvasive method of sample collection and simple DNA extraction from buccal swabs urine and hair using the phenol-chloroform method Buccal samples were subjected to DNA extraction immediately or after refrigeration (4–6C) for 3 days

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DNA RNA and Protein Extraction: The Past and The Present

30-11-2009Extraction of DNA RNA and protein is the basic method used in molecular biology These biomolecules can be isolated from any biological material for subsequent downstream processes analytical or preparative purposes In the past the process of extraction

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ExoCET: exonuclease in vitro assembly combined with

Digested genomic DNA was extracted with phenol–chloroform–isoamyl alcohol (25:24:1 pH 8 0) and precipitated with ethanol The DNA was dissolved in ddH 2 O and concentrated to 1 μg μl −1 End cut pipette tips were used to avoid shearing genomic DNA Ten micrograms of digested genomic DNA was used for ExoCET cloning

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Applied Biosystems SOLiD 3 System

This user guide is the proprietary material of Applied Biosystems LLC or its affiliates and is protected by laws of copyright The customer of the SOLiD ™ System is hereby granted limited non-exclusive rights to use this user guide solely for the purpose of operating the SOLiD System

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RNA PowerSoil Total RNA Isolation Kit

Phenol:Chloroform:Isoamyl Alcohol in the Hints and Troubleshooting Guide for preparation instructions) NOTE: Phenol and phenol:chloroform:isoamyl alcohol are subject to oxidation reactions that cause them to become yellow or pink colored which serves as an indicator that the phenol is NOT useable for RNA

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Applied Biosystems SOLiD 3 System

SOLiD™ SAGE™ Guide Applied Biosystems SOLiD Add 200 μL of UltraPure™ Phenol:Chloroform:Isoamyl Alcohol to the SAGE tags 2 Mix by high-speed vortexing and spin at 10 000 g for at least 5 minutes 3 Biosystems SOLiD™ 3 System Library Preparation Guide

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DNA RNA and Protein Extraction: The Past and The Present

Extraction of DNA RNA and protein is the basic method used in molecular biology These biomolecules can be isolated from any biological material for subsequent downstream processes analytical or preparative purposes In the past the process of extraction and purification of nucleic acids used to be complicated time-consuming labor-intensive and limited in terms of overall throughput

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Exonuclease combinations reduce noises in 3D genomics

A 1 2-fold volume of phenol/chloroform/isoamyl alcohol was used for DNA purification and the resultant DNA was analyzed by agarose gel electrophoresis Agencourt Ampure XP bead (Beckman Coulter) purification was carried out as an alternative purification method (Supplementary Figures S4 and S5) Data are presented as mean SEM

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Apoptosis DNA fragmentation analysis protocol

Find out the procedure for apoptosis DNA fragmentation in our useful step-by-step guide Print this protocol A distinctive biochemical feature of apoptosis is the fragmentation of DNA by a specific nuclease called caspase-activated DNase (CAD)

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PROTOCOLS

Preparation of DNA Microarray Printing Solution The DNA microarray printing solution consists of PCR products at a final concentration of greater than 100 ng/ m l (referably greater than 200 ng/ m l) For quality control all PCR reactions are evaluated on gels for size and number of bands

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